DNA Concentration Calculator

Calculate DNA concentration using absorbance at 260 nm, dilution factor, and path length.

Sample Name *

What would you like to calculate? *

A260 (Absorbance) *

Absorbance at 260 nm

Dilution Factor *

If undiluted, enter 1

Path Length (cm) *

Standard cuvette = 1.0 cm

Extinction Coefficient *

🧬 DNA Concentration Formula: Concentration = A260 × Dilution Factor × Extinction Coefficient / Path Length

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DNA Concentration

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Step-by-Step Calculation

Detailed solution process

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Concentration

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μg/mL

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A260

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Dilution Factor

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Ext. Coefficient

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μg/mL/A260

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Formula Reference

DNA concentration formulas

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DNA Concentration Tips

Understanding DNA quantification

🧬 Biological Disclaimer: DNA concentration calculations use the Beer-Lambert law. For pure DNA: A260 = 1.0 corresponds to ~50 μg/mL dsDNA. Results are for educational purposes.