DNA Melting Temperature Calculator (Tm)
Calculate the melting temperature (Tm) of DNA sequences using the Wallace rule or nearest-neighbor method.
🧬 Tm Formulas:
Wallace Rule: Tm = 2°C × (A+T) + 4°C × (G+C)
Basic Formula: Tm = 64.9 + 41 × (G+C - 16.4) / Length
Nearest-Neighbor: Tm = (ΔH × 1000) / (ΔS + R × ln(C/4)) - 273.15
Wallace Rule: Tm = 2°C × (A+T) + 4°C × (G+C)
Basic Formula: Tm = 64.9 + 41 × (G+C - 16.4) / Length
Nearest-Neighbor: Tm = (ΔH × 1000) / (ΔS + R × ln(C/4)) - 273.15
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Melting Temperature
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Step-by-Step Calculation
Detailed solution process
Tm
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°C
Length
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bp
GC Content
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%
Method
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Sequence Composition
Base counts and composition
Formula Reference
Tm calculation methods
Tm Tips
Understanding DNA melting temperature
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Biological Disclaimer: Tm calculations are estimates. Actual Tm can vary due to sequence context, buffer conditions, and instrument factors. For educational purposes.